Epidemiological and Molecular Evidence of Foodborne Poisoning Outbreak Caused by egc-Harboring Staphylococcus aureus of new sequence type 7591

ObjectiveThis paper presented a detailed analysis of the epidemiology and molecular characteristics staphylococcal food poisoning (SFP) that occurred in hotel Hangzhou.MethodsA total 46 guests at underwent an epidemiological survey. Samples stool from patients, vomit, swabs kitchen, leftover items, anal handlers were taken investigated for presence potential pathogenic bacteria. Molecular techniques Whole Genome Sequencing (WGS) performed to track evolution S. aureus associated with outbreak SFP.ResultsForty-six individuals displayed gastrointestinal symptoms. Seventeen isolates discovered carry seg, sei, sem, sen, seo, selu genes found specific enterotoxin gene cluster (egc) operon, but without classical enterotoxins such as SEA ∼ SEE. All egc-positive shared identical PFGE profiles classified under new ST7591 (Clonal Complex 72) spa typing t148. In addition, some obtained sources sold Hangzhou over past three years carrying egc grouped ST72 lineage (CC72). Through WGS, strong genetic connection was revealed between these clinical China.ConclusionsS. non-classical suggested be cause SFP humans.